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Baculoviral expressed HLA class I heavy chains used to screen a synthetic peptide library for allele-specific peptide bi...

Smith, Mh Nuara, Aa Egen, Jg Sirjani, Db Kit S. Lam Grimes, Wj

Recombinant baculoviruses encoding truncated HLA-A*0101 and HLA-A*0201 class I heavy chains have been isolated and used to infect lepidopteran cells. Proteins overexpressed in this system were glycosylated, and consisted of 282 amino acid residues after signal sequence cleavage. These class I heavy chains could fold into their native conformation i...

Molecular cloning, expression and mutagenesis of an anti-insulin single chain Fv (scFv).

Lake, Df Kit S. Lam Peng, L Hersh, Em

Published in Molecular Immunology

The immunoglobulin variable region genes of a murine anti-insulin IgG-producing hybridoma were rescued and cloned into a bacterial expression vector. The variable regions of the gamma heavy chain and the kappa light chain were expressed independently and together as a single chain antibody (scFv). The variable heavy chain alone demonstrated the abi...

Peptide sequences binding to MHC class I proteins.

Smith, Mh Kit S. Lam Hersh, Em Lebl, M Grimes, Wj

Published in Molecular Immunology

Motifs for peptides which bind specifically to the human class I major histocompatibility complex molecules HLA-A2 and B7 were determined by sequence analysis of class I-bound peptides selected from a random synthetic library of nonamers. Thirteen individual peptides were sequenced for HLA-A2, twelve individual and nine pooled peptides were sequenc...

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Baculoviral expressed HLA class I heavy chains used to screen a synthetic peptide library for allele-specific peptide bi...

Molecular cloning, expression and mutagenesis of an anti-insulin single chain Fv (scFv).

Peptide sequences binding to MHC class I proteins.